The Ultrastructure of Melanoma Cells Treated with 5-Bromodeoxyuridine
نویسندگان
چکیده
منابع مشابه
Suppression of Pigmentation in Mouse Melanoma Cells by 5-bromodeoxyuridine
Low concentrations (1-3 microg/ml) of 5-bromodeoxyuridine (BrdU) reversibly suppress pigmentation in a highly pigmented clone (B(5)59) of cultured B16 mouse melanoma cells. We have found that unpigmented cells (clone C(3)471), derived by long-term culture of B(5)59 cells in 1 microg of BrdU/ml, were completely amelanotic with no biochemically or cytochemically detectable tyrosinase activity or ...
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The thymidine analog 5-bromodeoxyuridine (BrUdR) incorporated into DNA has been successfully used as a selective agent (1, 2) or mutagen (3) on various animal cells. Some of its effects are understandable in terms of base substitution in the genetic material; others, however, such as the reversible suppression of cytodifferentiation (4-7), remain unexplained. We have examined the effects of pro...
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B16 mouse melanoma cells (clone BS59) grown in 1 to 3 ¿ig of 5-bromodeoxyuridine (BUdR) per ml form few or no tumors in C57BL/6J adult mice when injected subcutaneously in doses at which untreated cells always form tumors. Injections of BUdR-grown cells protect adult mice against melanoma. Melanoma cells grown in 1/ig of BUdR per ml for almost 1 year (clone C3471) retain a plating efficiency s...
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The ultrastructure of chromosomes from tissue culture cells blocked in division with vincristine sulphate or colchicine was examined. Kinetochores had a distinctive morphology consisting of trilaminar dense plates closely applied to the chromosomes. In longitudinal sections, kinetochores appeared as separate plates attached to opposite sides of the chromosomes. In transverse sections of some pr...
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5-Bromodeoxyuridine induces the differentiation of mouse neuroblastoma C1300 to cells that morphologically resemble mature neurons. The induced differentiation can take place in the absence of DNA synthesis. This suggests that the halogenated pyrimidine need not be incorporated into DNA to alter the phenotype of the cell.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1974
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12679938